OPTOGENETIC ARRAYS

Opto-Microelectrodes Array

With our novel and reliable products the photoactivation/photoinhibition of cells through an integrated optical fiber, multichannel electrophysiological recording and/or stimulation of an extended brain area can be achieved in parallel.

CHARACTERISTICS

  • Multiple optical fiber diameter available.
  • Up to 32 penetrating electrodes surrounding one or more optical fiber.
  • Custom length of each electrode and optical fibers between 1 to 40 mm, including different lengths within the array.
  • Custom spacing between electrodes is in the range of 0.1 - 1 mm
  • Custom microelectrode impedance for individual electrodes ranges between 10 kΩ - 5 MΩ.
  • Custom electrode tip diameter between 1 - 6 µm.
  • Custom reference and ground electrodes according to the researcher’s requirements.
  • Optionally integrated fiber optic and/or drug delivery cannula.
  • Parylene-C electrode insulation.
  • Pure Iridium is suggested for chronic stimulation studies.
  • Optional polyimide tubing for additional stiffness.

ELECTRODE METAL TYPE

Pt-Ir
Platinum
Iridium
Ir
Pure Iridium

ONLINE DESIGN FORMS

APPLICATIONS

Single Unit
LFP ECoG
Spinal
Cord
Isolated
Preparations

SUBJECTS

Primate
PRIMATES
Rat
RAT-MICE
Birds
BIRDS
Feline
FELINE

The fiber optic is positioned in the middle of the array, its vertical position is custom and its receptacle is compatible with most of the light sources.

The small-scale but high-density arrays provide multichannel Single-Unit (SUA), Multi-Unit (MUA) and Field Potential (FP) data leading to consistent and high-quality results.

STANDARD LAYOUTS

Layout I. (Top view)
Layout I. (Top view)
  • Electrodes (gray dots) are 75 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Layout II. (Top view)
Layout II. (Top view)
  • Electrodes (gray dots) are 75 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Layout III. (Top view)
Layout III. (Top view)
  • Electrodes (gray dots) are 125 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Layout IV. (Top view)
Layout IV. (Top view)
  • Electrodes (gray dots) are 125 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Optogenetics combines genetic targeting of specific neurons

Optogenetics combines genetic targeting of specific neurons or proteins with optical technology for imaging or control of the targets within intact, living neural circuits (Deisseroth et al., Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci. 2006 Oct 11;26(41):10380-6.)

Data provided by IIka Diester

Data provided by IIka Diester, Sensory-motor circuits and Optogenetics, Ernst Struengmann Institute in Cooperation with Max Planck Society - Germany

Opto Micro-Wire Array

With our novel and reliable products the photoactivation/photoinhibition of cells through an integrated optical fiber, multichannel electrophysiological recording and/or stimulation of an extended brain area can be achieved in parallel.

Designed for chronic experiments, the MicroProbes Opto-MWA provides the optimum in flexibility and affordable alternative for the neuroscience community.

CHARACTERISTICS

  • Multiple optical fiber diameter available.
  • Up to 32 penetrating wires per array for short- and long-term recording.
  • Custom wire length (5-23 mm), including different length for each row.
  • Custom spacing between electrodes is in the range of 0.1 - 1 mm
  • Custom spacing between wires is in the range of 250 - 1000 µm.
  • Custom wire diameter options available (25/ 50 µm).
  • Impedance options between 200-800 kΩ.
  • Stainless Steel and Platinum/Iridium fine wires teflon or polyimide insulation material.
  • Custom reference and ground electrode according to the researcher's requeriments.

ELECTRODE METAL TYPE

Pt-Ir
Platinum
Iridium
SS
Stainless
Steel

ONLINE FORMS

APPLICATIONS

Single Unit
LFP ECoG
Spinal
Cord
Isolated
Preparations

SUBJECTS

Primate
PRIMATES
Rat
RAT-MICE
Birds
BIRDS
Feline
FELINE

The fiber optic is positioned in the middle of the array, its vertical position is custom and its receptacle is compatible with most of the light sources.

img comparativa

The small-scale but high-density arrays provide multichannel Single-Unit (SUA), Multi-Unit (MUA) and Field Potential (FP) data leading to consistent and high-quality results.

STANDARD LAYOUTS

Layout I. (Top view)
Layout I. (Top view)
  • Electrodes (gray dots) are 25 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Layout II. (Top view)
Layout II. (Top view)
  • Electrodes (gray dots) are 25 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Layout III. (Top view)
Layout III. (Top view)
  • Electrodes (gray dots) are 50 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Layout IV. (Top view)
Layout IV. (Top view)
  • Electrodes (gray dots) are 50 µm in diameter.
  • The blue dot is the optical fiber, which is 230 µm in diameter.
  • R = reference electrode
Example of Layout III. (Top view)
Optogentics MWA Layout III zoom
Optogenetics combines genetic targeting of specific neurons

Optogenetics combines genetic targeting of specific neurons or proteins with optical technology for imaging or control of the targets within intact, living neural circuits (Deisseroth et al., Next-generation optical technologies for illuminating genetically targeted brain circuits. J Neurosci. 2006 Oct 11;26(41):10380-6.)

Data provided by IIka Diester, Sensory-motor circuits and Optogenetics, Ernst Struengmann Institute in Cooperation with Max Planck Society - Germany

Recording of an optically stimulated neuron

Recording of an optically stimulated neuron expressing channel rhodopsin (CHR2) in the striatum of a mouse using a custom Opto Micro-Wire array (Microprobes - Gaithersburg, MD). A. RASTER and PETH of striatal spikes generated using 1ms pulses of 473nm blue laser light. B. Waveforms of spikes from A, plotted in relation to the onset and offset of the laser pulse. C. Spike fidelity is linearly correlated to laser output intensity.

Courtesy of Mark O. West’s Lab at Rutgers University - www.rci.rutgers.edu/~markwest

CUSTOMERS FEEDBACK

I've been a customer of Microprobes for Life Science for years and my lab has been using their products for even longer. Their microwire arrays and optogenetic-microwire arrays have always worked extremely well for us. We have used them to record in a variety of brain areas in rats and mice at both acute and chronic time points. The electrodes have always been in pristine condition, made to our exact specifications.
Eric Emmons
Eric EmmonsNarayanan Lab

GALLERY

Opto-MEA
Opto-MWA